Altair-dvOPM: an open-access platform for large-field three-dimensional tissue imaging | bioRxiv
Altair-dvOPM is an open-access direct-view oblique plane microscope designed for large-field, three-dimensional imaging of cleared and expanded tissue sections. By combining photographic-lens-based detection, externally launched oblique illumination and precision-registered modular baseplates, the system achieves micrometer-scale lateral resolution over a ~5.4 mm field of view without custom objectives or highly specialized alignment procedures. We demonstrate imaging across scales, from subcellular structures in expanded cells to centimeter-scale expanded tissue sections, and provide documentation, CAD files, Zemax models and open-source control software to support replication and extension. K.M.D. is a founder of Discovery Imaging Systems, LLC. K.M.D. and R.F. hold a patent on ASLM and have consultancy agreements with 3i, Inc. (Denver, CO, USA). H.Z. is a co-founder of Quotient Therapeutics and Jumble Therapeutics, and serves as an advisor to NewLimit. Reviews and Context 0 Comment 0
New Results , View ORCID ProfileMuaaz Faiyazuddin, View ORCID ProfileTrung Duc Nguyen, View ORCID ProfileJohn Haug, View ORCID ProfileQionghua Shen, View ORCID ProfileSeweryn Gałecki, View ORCID ProfileHazel M. Borges, View ORCID ProfileBingying Chen, Xiaoding Wang, View ORCID ProfileHao Zhu, View ORCID ProfileSamuel S. Pappas, View ORCID ProfileFabian F. Voigt, View ORCID ProfileReto Fiolka, View ORCID ProfileKevin M. Dean doi: https://doi.org/10.64898/2026.05.08.723912 Abstract Altair-dvOPM is an open-access direct-view oblique plane microscope designed for large-field,…
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