A hybrid open-top light-sheet microscope for versatile multi-scale imaging of cleared tissues | Nature Methods
A ‘hybrid’ open-top light-sheet microscope is described, which can be used for broad multi-scale volumetric imaging of one or more large tissues, cleared with diverse protocols, and conveniently mounted on an array of sample holders.
Data availability The customized ZEMAX files, CAD files, list of components, and a summary of all datasets and the associated imaging parameters are available as Supplementary Data. Due to the large size of the imaging datasets collected within this manuscript, the datasets are not available in a public repository and are available from the authors upon request. Code availability The simulation codes used to model the lateral and axial resolution of the various microscope architectures is available on GitHub. The acquisition software code for the microscope is available from the authors…
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