flâneur

Expansion microscopy of C. elegans

elifesciences.org · 43,823 words · saved by 1 readers

We recently developed expansion microscopy (ExM), which achieves nanoscale-precise imaging of specimens at ~70 nm resolution (with ~4.5x linear expansion) by isotropic swelling of chemically processed, hydrogel-embedded tissue. ExM of C. elegans is challenged by its cuticle, which is stiff and impermeable to antibodies. Here we present a strategy, expansion of C. elegans (ExCel), to expand fixed, intact C. elegans. ExCel enables simultaneous readout of fluorescent proteins, RNA, DNA location, and anatomical structures at resolutions of ~65–75 nm (3.3–3.8x linear expansion). We also developed epitope-preserving ExCel, which enables imaging of endogenous proteins stained by antibodies, and iterative ExCel, which enables imaging of fluorescent proteins after 20x linear expansion. We demonstrate the utility of the ExCel toolbox for mapping synaptic proteins, for identifying previously unreported proteins at cell junctions, and for gene expression analysis in multiple individual neurons of

Expansion microscopy of C. elegans | eLife Version of Record May 1, 2020 Download Cite Share Comment Open annotations (there are currently 0 annotations on this page). 17,728 views 1,787 downloads 94 citations Altmetric provides a collated score for online attention across various platforms and media. See more details Share this article Doi Copy to clipboard Cite this article Chih-Chieh (Jay) Yu Nicholas C Barry Asmamaw T Wassie Anubhav Sinha Abhishek Bhattacharya Shoh Asano Chi Zhang Fei Chen Oliver Hobert Miriam B Goodman Gal Haspel Edward S Boyden (2020) Expansion microscopy of C. elegans e

saved by

related reading