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Light sheet microscopy – Huisken Lab

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Creating tailor-made optical microscopes to non-invasively explore morphogenesis and function in living organisms. In every implementation of fluorescence microscopy, we want to illuminate a specimen with light of a specific wavelength to excite fluorophores, and then collect the emitted photons by eye or with a detector. Typically, illumination and detection share a part of the microscope’s beam path – at least the objective lens. Hence, illumination occurs from the same direction as detection, resulting in a large part of the specimen being illuminated. Fluorescence microscopy requires powerful illumination, which can have detrimental impacts on specimens, namely photo-bleaching and photo-toxicity. Thus, it is much more effective to limit illumination to the parts of the sample that are actually being imaged. Light sheet microscopy is an elegant implementation of this idea. The principle of light sheet microscopy – also known as selective plane illumination microscopy (SPIM) – is to

Basic Principles In every implementation of fluorescence microscopy, we want to illuminate a specimen with light of a specific wavelength to excite fluorophores, and then collect the emitted photons by eye or with a detector. Typically, illumination and detection share a part of the microscope’s beam path – at least the objective lens. Hence, illumination occurs from the same direction as detection, resulting in a large part of the specimen being illuminated. Fluorescence microscopy requires powerful illumination, which can have detrimental impacts on specimens, namely photo-bleach

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