Two-Photon Excitation Microscopy for the Study of Living Cells and Tissues - PMC
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. Author manuscript; available in PMC: 2014 Jun 1. Published in final edited form as: Curr Protoc Cell Biol. 2013 Jun;0 4:Unit–4.1124. doi: 10.1002/0471143030.cb0411s59 Abstract Two-photon excitation microscopy is an alternative to confocal microscopy that provides advantages for three-dimensional and deep tissue imaging. This unit will describe the basic physical principles behind two-photon excitation and discuss the advantages and limitations of its use in laser-scanning microscopy. The principal advantages of two-photon microscopy are reduced phototoxicity, increased imaging depth,…
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