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Two-Photon Excitation Microscopy for the Study of Living Cells and Tissues - PMC

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The .gov means it’s official. Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site. The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely. Preview improvements coming to the PMC website in October 2024. Learn More or Try it out now. 1University of Colorado, Anschutz Medical Campus, Aurora, Colorado 2Vanderbilt University Medical Center, Nashville, Tennessee Two-photon excitation microscopy is an alternative to confocal microscopy that provides advantages for three-dimensional and deep tissue imaging. This unit will describe the basic physical principles behind two-photon excitation and discuss the advantages and limitations of its use in laser-scanning microscopy. The principal advant

. Author manuscript; available in PMC: 2014 Jun 1. Published in final edited form as: Curr Protoc Cell Biol. 2013 Jun;0 4:Unit–4.1124. doi: 10.1002/0471143030.cb0411s59 Abstract Two-photon excitation microscopy is an alternative to confocal microscopy that provides advantages for three-dimensional and deep tissue imaging. This unit will describe the basic physical principles behind two-photon excitation and discuss the advantages and limitations of its use in laser-scanning microscopy. The principal advantages of two-photon microscopy are reduced phototoxicity, increased imaging depth,…

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