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Miniscope3D: optimized single-shot miniature 3D fluorescence microscopy | Light: Science & Applications

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Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript. Advertisement Light: Science & Applications volume  9, Article number: 171 (2020) Cite this article 17k Accesses 79 Citations 48 Altmetric Metrics details An Author Correction to this article was published on 17 April 2023 This article has been updated Miniature fluorescence microscopes are a standard tool in systems biology. However, widefield miniature microscopes capture only 2D information, and modifications that enable 3D capabilities increase the size and weight and have poor resolution outside a narrow depth range. Here, we achieve the 3D capability by replacing the tube lens of a conventional 2D Miniscope with an

System theory Miniscope3D encodes volumetric information via a thin phase mask placed at the aperture stop of the gradient index (GRIN) objective lens (see Fig. 1). The goal of our design is to optimize the microscope optics for compressed sensing, enabling the capture of a large number of voxels from a small number of sensor pixels. To achieve this, the phase mask comprises an engineered pattern of multifocal microlenses, designed such that each fluorescent point source in the scene produces a unique high-frequency pattern of focal spots at the sensor plane, thus encoding its 3D position.…

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