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Technologies to measure and modulate protein subcellular localization | Nature Reviews Molecular Cell Biology

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Subcellular localization is important for protein function. This Review discusses advances in spatial subcellular proteomics, assesses the strengths and weaknesses of technologies that measure and control protein localization, and considers their potential uses in cell biology and medicine.

Thul, P. J. et al. A subcellular map of the human proteome. Science 356, eaal3321 (2017). Article PubMed Google Scholar Geladaki, A. et al. Combining LOPIT with differential ultracentrifugation for high-resolution spatial proteomics. Nat. Commun. 10, 331 (2019). Article PubMed PubMed Central Google Scholar Sigaeva, A., Hutchings, C., Cesnik, A., Lilley, K. S. & Lundberg, E. Subcellular localization as a driver of protein function. Nat. Rev. Mol. Cell Biol. https://doi.org/10.1038/s41580-026-00947-3 (2026). Christopher, J. A. et al. Subcellular proteomics. Nat. Rev. Methods Primers 1,…

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