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TGP, an extremely stable, non-aggregating fluorescent protein created by structure-guided surface engineering - PMC

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In this paper we describe the engineering and X-ray crystal structure of Thermal Green Protein (TGP), an extremely stable, highly soluble, non-aggregating green fluorescent protein. TGP is a soluble variant of the fluorescent protein eCGP123, which ...

. Author manuscript; available in PMC: 2016 Jul 1. Published in final edited form as: Proteins. 2015 May 8;83(7):1225–1237. doi: 10.1002/prot.24699 Abstract In this paper we describe the engineering and X-ray crystal structure of Thermal Green Protein (TGP), an extremely stable, highly soluble, non-aggregating green fluorescent protein. TGP is a soluble variant of the fluorescent protein eCGP123, which despite being highly stable, has proven to be aggregation-prone. The X-ray crystal structure of eCGP123, also determined within the context of this paper, was used to carry out rational…

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