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Spontaneous and indefinite blinking in upconverting nanoparticles for ångström-precision multicolour super-resolution imaging | Nature Nanotechnology

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Compositionally engineered, spontaneously blinking upconverting nanoparticles enable multicolour super-resolution imaging with a single excitation source and achieve localization precision down to 0.62 Å for single-molecule imaging on cell membranes.

Download PDF Abstract Single-molecule localization microscopy enables high-resolution biological imaging, but its precision is limited by the rapid photobleaching of conventional fluorophores. Multicolour imaging is further constrained by the need for spectrally distinct dyes requiring separate excitations or sequential acquisition. Here we show that small (~10 nm) upconverting nanoparticles can be compositionally tuned to exhibit spontaneous, sustained blinking under single near-infrared excitation without optical or chemical modulation. By adjusting sensitizer (Yb 3+ )–emitter (Tm 3+ /Er 3+

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