Fat2 polarizes the WAVE complex in trans to align cell protrusions for collective migration | eLife

For a group of cells to migrate together, each cell must couple the polarity of its migratory machinery with that of the other cells in the cohort. Although collective cell migrations are common in animal development, little is known about how protrusions are coherently polarized among groups of migrating epithelial cells. We address this problem in the collective migration of the follicular epithelial cells in Drosophila melanogaster. In this epithelium, the cadherin Fat2 localizes to the trailing edge of each cell and promotes the formation of F-actin-rich protrusions at the leading edge of the cell behind. We show that Fat2 performs this function by acting in trans to concentrate the activity of the WASP family verprolin homolog regulatory complex (WAVE complex) at one long-lived region along each cell’s leading edge. Without Fat2, the WAVE complex distribution expands around the cell perimeter and fluctuates over time, and protrusive activity is reduced and unpolarized. We further

Fat2 polarizes the WAVE complex in trans to align cell protrusions for collective migration | eLife Version of Record October 17, 2022 Accepted Manuscript September 26, 2022 Download Cite Share Comment Open annotations (there are currently 0 annotations on this page). 2,044 views 333 downloads 35 citations Altmetric provides a collated score for online attention across various platforms and media. See more details Share this article Doi Copy to clipboard Cite this article Audrey Miller Williams Seth Donoughe Edwin Munro Sally Horne-Badovinac (2022) Fat2 polarizes the WAVE complex in trans to a

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