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Super-resolved analysis of colocalization between replication and transcription along the cell cycle in a model of oncogene activation | Communications Biology

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A super-resolution imaging method for cell cycle sorting reveals that the PML-RARα oncogene induces an increase in colocalization between replication and transcription in early S phase.

Download PDF Subjects Nanoscale biophysics Oncogenes Super-resolution microscopy Abstract To understand how oncogenes affect genome organization, it is essential to visualize fundamental processes such as DNA replication and transcription at high resolution in intact cells. At the same time, it is important to determine the progression of the cell along the cell cycle, as cell cycle regulation is crucial for the control of cell proliferation and oncogenesis. Here, we present a super-resolution imaging-based method to analyze single cell nuclei sorted according to specific phases of the cell cy

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