Rapid immunoprecipitation mass spectrometry of endogenous proteins (RIME) for analysis of chromatin complexes | Nature Protocols
This protocol describes affinity purification of endogenous protein complexes for mass spectrometry analysis. Optimized to study formaldehyde-crosslinked proteins isolated by chromatin immunoprecipitation, it can be adapted to study other protein complexes.
Subjects Chromatin Immunoprecipitation Mass spectrometry Proteomics Abstract Rapid immunoprecipitation mass spectrometry of endogenous protein (RIME) is a method that allows the study of protein complexes, in particular chromatin and transcription factor complexes, in a rapid and robust manner by mass spectrometry (MS). The method can be used in parallel with chromatin immunoprecipitation–sequencing (ChIP-seq) experiments to provide information on both the cistrome and interactome for a given protein. The method uses formaldehyde fixation to stabilize protein complexes. By using antibodies aga
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