High-throughput measurements of protein domain functions using magnetic separation - PubMed
The .gov means it’s official. Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site. The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely. High-throughput screening of protein domains enables the systematic discovery of protein sequences that encode specific cellular functions. Fluorescence-activated cell sorting-based assays have long been the standard readout for such screens but remain time- and resource-intensive, imposing practical limits on library size and coverage. Here we describe a scalable magnetic separation-based workflow that provides an alternative to fluorescence-activated cell sorting for screening large protein libraries in mammalian cells. We engineered a modular sy
Construction of a versatile expression library for all human single-pass transmembrane proteins for receptor pairings by high throughput screening. Yang W, Padkjær SB, Wang J, Sun Z, Shan B, Yang L, Chen H, Kang L, Madsen D, Li X, Shen C, Yu B, Zhu H, Chao TY, Cao Z, Li D, Liu W, Du Y, Xu J, Hao D, Xu F, Peng L, Li T, Wang L, Li L, Xing H, Liu D, Liu Z, Guan Z, Wang W, Cheng H, Østergaard H, Chang C, Yang Z, Boel E, Su J.Yang W, et al.J Biotechnol. 2017 Oct 20;260:18-30. doi: 10.1016/j.jbiotec.2017.08.023. Epub 2017 Sep 1.J Biotechnol. 2017.PMID: 28867483
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