Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection - PMC
We have systematically made a set of precisely defined, single-gene deletions of all nonessential genes in Escherichia coli K-12. Open-reading frame coding regions were replaced with a kanamycin cassette flanked by FLP recognition target sites by ...
Mol Syst Biol . 2006 Feb 21;2:2006.0008. doi: 10.1038/msb4100050 Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection Tomoya Baba Tomoya Baba 1 Institute for Advanced Biosciences, Keio University, Tsuruoka City, Yamagata, Japan 2 Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara, Japan Find articles by Tomoya Baba 1, 2 , Takeshi Ara Takeshi Ara 1 Institute for Advanced Biosciences, Keio University, Tsuruoka City, Yamagata, Japan Find articles by Takeshi Ara 1 , Miki Hasegawa Miki Hasegawa 1 Institute for A
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