A systematic CRISPR screen defines mutational mechanisms underpinning signatures caused by replication errors and endogenous DNA damage | Nature Cancer
Nik-Zainal and colleagues leverage CRISPR–Cas9 and whole-genome sequencing to examine mutational patterns following knockout of 42 human DNA repair genes. They further develop and validate a clinically relevant tool to detect mismatch repair-deficient tumors.
Data availability Raw sequence files are deposited at the European Genome-Phenome Archive with accession numbers EGAS00001000800 and EGAS00001000874. Mutation calls have been deposited at Mendeley: https://doi.org/10.17632/ymn3ykkmyx. hiPSCs can be obtained directly from the authors. The curated data are available for general browsing from our reference mutational signatures website, Signal (https://signal.mutationalsignatures.com). Age information relating to human patient samples is not publicly available as this could compromise privacy and lead to identification of the individuals.…
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