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High-speed, cortex-wide volumetric recording of neuroactivity at cellular resolution using light beads microscopy | Nature Methods

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Light beads microscopy is a two-photon microscopy approach that allows high-speed volumetric imaging of neuronal activity at the mesoscale.

Subjects Fluorescence imaging Mouse Multiphoton microscopy Neuroscience Optical imaging A Publisher Correction to this article was published on 08 November 2021 This article has been updated Abstract Two-photon microscopy has enabled high-resolution imaging of neuroactivity at depth within scattering brain tissue. However, its various realizations have not overcome the tradeoffs between speed and spatiotemporal sampling that would be necessary to enable mesoscale volumetric recording of neuroactivity at cellular resolution and speed compatible with resolving calcium transients. Here, we introd

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