Imaging neuronal voltage beyond the scattering limit | Nature Methods

ALI is an approach for analyzing voltage imaging data that is inspired by algorithms used in super-resolution microscopy. It allows resolving the activity of single neurons in densely labeled populations in scattering conditions.

Main Neurons compute and communicate using brief electrical signals known as APs. Lasting only about 1 ms, these signals carry rich information about our perception, actions and thoughts. Voltage-sensitive fluorescent proteins1,2,3,4,5,6,7,8,9 convert APs into rapid changes in fluorescence intensity, providing a means to measure these signals in all expressing neurons. However, challenges persist in extracting this information from dense neuronal populations in vivo. Imaging voltage signals in densely packed neurons requires high resolution in both space and time. While two-photon…

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• Mackenzie Dion

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