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organoid morphodynamics | Living Matter Lab

tzerhan.com · 397 words · saved by 1 readers

Collective cell dynamics and morphogenesis play a crucial role in many developmental and physiological contexts. In this work (bioRxiv 2022), we use murine pancreas-derived organoids to study tissue rotation, a multicellular phenomenon widely reported both in vivo and in vitro. Combining light sheet microscopy and custom-built computational framework, we quantify spatiotemporal tissue flows in 3D. Using a 3D vertex model, we demonstrate how the interplay between traction force and polarity alignment can account for distinct rotational dynamics observed in experiments. Our results show that the spherical tissue rotates as an active solid and exhibits spontaneous chiral symmetry breaking. Using a continuum model, we demonstrate how the types and location of topological defects in the polarity field underlie this symmetry breaking process. Altogether, our work shows that tissue chirality can arise via topological defects in the pattern of cell traction forces, with potential implications

--> organoid morphodynamics | Living Matter Lab top of page Collective cell dynamics and morphogenesis in organoids Collective cell dynamics and morphogenesis play a crucial role in many developmental and physiological contexts. In this work ( bioRxiv 2022 ), we use murine pancreas-derived organoids to study tissue rotation, a multicellular phenomenon widely reported both in vivo and in vitro . Combining light sheet microscopy and custom-built computational framework, we quantify spatiotemporal tissue flows in 3D. Using a 3D vertex model, we demonstrate how the interplay between traction force

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