The Mechanical Influence of Densification on Initial Epithelial Architecture | bioRxiv

Epithelial tissues are the most abundant tissue type in animals, lining body cavities and generating compartment barriers. The function of a monolayer epithelium – whether protective, secretory, absorptive, or filtrative –relies on regular tissue architecture with respect to the apical-basal axis. Using an unbiased 3D analysis pipeline developed in our lab, we previously showed that epithelial tissue architectures in culture can be divided into distinct developmental categories, and that these are intimately connected to cell density: at sparse densities, cultured epithelial cell layers have a squamous morphology (Immature); at intermediate densities, these layers develop lateral cell-cell borders and rounded cell apices (Intermediate); cells at the highest densities reach their full height and demonstrate flattened apices (Mature). These observations prompted us to ask whether epithelial architecture emerges from the mechanical constraints of densification, and to what extent a hallmark feature of epithelial cells, namely cell-cell adhesion, contributes. In other words, to what extent is the shape of cells in an epithelial layer a simple matter of sticky, deformable objects squeezing together? We addressed this problem using a combination of computational modeling and experimental manipulations. Our results show that the first morphological transition, from Immature to Intermediate, can be explained simply by cell crowding. Additionally, we identify a new division (and thus transition) within the Intermediate category, and find that this second morphology relies on cell-cell adhesion. ### Competing Interest Statement The authors have declared no competing interest.

The Mechanical Influence of Densification on Initial Epithelial Architecture | bioRxiv Skip to main content New Results The Mechanical Influence of Densification on Initial Epithelial Architecture View ORCID Profile Christian Cammarota , View ORCID Profile Nicole S. Dawney , Phillip M. Bellomio , Maren Jüng , View ORCID Profile Alexander G. Fletcher , View ORCID Profile Tara M. Finegan , View ORCID Profile Dan T. Bergstralh doi: https://doi.org/10.1101/2023.05.07.539758 Christian Cammarota 1 Department of Physics and Astronomy, University of Rochester , Rochester, NY, USA Find this author on G

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