All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins | Nature Methods

A combination of a sensitive blue light–gated channelrhodopsin actuator and red-shifted Arch-based voltage sensors allows all-optical electrophysiology without cross-talk in cultured neurons or brain slices.

Peron, S. & Svoboda, K. From cudgel to scalpel: toward precise neural control with optogenetics. Nat. Methods 8, 30–34 (2011). Article CAS PubMed Google Scholar Petreanu, L., Mao, T., Sternson, S.M. & Svoboda, K. The subcellular organization of neocortical excitatory connections. Nature 457, 1142–1145 (2009). Article CAS PubMed PubMed Central Google Scholar Scanziani, M. & Hausser, M. Electrophysiology in the age of light. Nature 461, 930–939 (2009). Article CAS PubMed Google Scholar Boulting, G.L. et al. A functionally characterized test set of human induced pluripotent stem cells.…

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