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Two-photon microscopy using fiber-based nanosecond excitation

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Sebastian Karpf,1,2 Matthias Eibl,3 Benjamin Sauer,3 Fred Reinholz,3 Gereon Hüttmann,3 and Robert Huber1,3,* 1Lehrstuhl für BioMolekulare Optik, Fakultät für Physik, Ludwig-Maximilians-Universität München, Oettingenstr. 67, 80538 Munich, Germany 2Department of Electrical Engineering, University of California, Los Angeles, California 90095, USA 3Institut für Biomedizinische Optik, Universität zu Lübeck, Peter-Monnik-Weg 4, 23562 Lübeck, Germany *Corresponding author: robert.huber@bmo.uni-luebeck.de Two-photon excitation fluorescence (TPEF) microscopy is a powerful technique for sensitive tissue imaging at depths of up to 1000 micrometers. However, due to the shallow penetration, for in vivo imaging of internal organs in patients beam delivery by an endoscope is crucial. Until today, this is hindered by linear and non-linear pulse broadening of the femtosecond pulses in the optical fibers of the endoscopes. Here we present an endoscope-ready, fiber-based TPEF microscope, using nanose

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