flâneur

Sam Brook

3 followers · 22 following · 349 views

on the atlas — 79

highlights — 9

  • ELP fusions can be purified using ITC, and the ELP can then be removed using a proteolytic cleavage site or a self-cleaving intein—a self-splicing protein domain—that has been inserted between the ELP and target protein
    Elastin-Like Polypeptides for Biomedical Applications | Annual Review of Biomedical Engineering
  • We ascribe value to the time we spend discovering things online
    Notes apps are where ideas go to die. And that’s good. · reproof
  • We’ll exploit this using ION EXCHANGE CHROMATOGRAPHY (IEX), where we bind proteins to resins that are oppositely-charged. Ions are charged things and basically you “exchange” ions from salts (like the Na+ or Cl- of NaCl (table salt) with protein ions. Then you can gradually increase the salt concentrations so that those salt ions outcompete the protein and you get another exchange. Or you can change the pH to change the protein’s overall charge (the lower the pH, the more free H+ for the protein to latch onto -> become more positive & vice versa) In CATION EXCHANGE chromatography you have nega…
    Protein purification workflow – The Bumbling Biochemist
  • Universities, IIRC, started when students banded together to pay lecturers. That might be a more fruitful approach than this one.
    2:00PM Water Cooler 11/9/2021 | naked capitalism
  • Achieving a research-level understanding of most topics is like climbing a mountain. Aspiring researchers must struggle to understand vast bodies of work that came before them, to learn techniques, and to gain intuition. Upon reaching the top, the new researcher begins doing novel work, throwing new stones onto the top of the mountain and making it a little taller for whoever comes next.
    Curius
  • Similar to aspartic acid, the amino acid proline has the rare property of being able to occupy both cis and trans isomers of its prolyl peptide bonds with ease
    Proline isomerization in epigenetics
  • The discoveries of recombinant DNA and gene synthesis transformed biologists into engineers, as they finally gained “write access” to the code that underlies biological systems.
    The future of biotech is founder-led | Tony Kulesa | Pillar VC
  • Links represent a threat to closed systems.
    Adactio: Links—Tagged with links
  • uld find about business. The subject that interests him, he’s read newspap
    Curius / Onboarding