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Expanding the human proteome with microproteins and peptideins | Nature

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A large-scale proteomics analysis of the dark proteome by the TransCODE Consortium reveals many translated non-canonical open reading frames to encode microproteins and peptideins.

Download PDF Subjects Evolutionary biology Gene expression Genetic databases Open reading frames Protein databases Abstract A major scientific drive is to characterize the protein-coding genome, which is a primary basis for studying human health. But the fundamental question remains of what has been missed in previous analyses. Over the past decade, the translation of non-canonical open reading frames (ncORFs) has been observed across human cell types and disease states 1 , 2 , 3 , with major implications for biomedical science. However, a key gap in knowledge has been which ncORFs produce sma

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