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BLAST Tutorial | Texas A&M Institute for Genomic Medicine

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BLAST (Basic Local Alignment Search Tool) is a set of programs designed to perform similarity searches against a database of sequences. Scientists frequently use such searches to gain insight into evolutionary relationships and use that to infer function and biological importance of gene products. BLAST uses an algorithm that seeks out local alignment (the alignment of some portion of two sequences) as opposed to global alignment (the alignment of two sequences over their entire length). By searching for local alignments, BLAST is able to identify regions of similarity within two sequences. Since more than one codon or triplet of nucleotides could code for a particular amino acid, a considerable variation in nucleotide sequences could translate into the same amino acid sequence. Comparing amino acid sequences is a more reliable predictor of similarity between two sequences than comparing nucleotide sequences. For this reason, this tutorial will focus on using blastp to compare the gene

tag based on what is being viewed. We filter the output of wp_title() a bit - see agriflex_filter_wp_title() in functions.php. --> BLAST Tutorial | Texas A&M Institute for Genomic Medicine tag of your theme, or you will break many plugins, which generally use this hook to add elements to such as styles, scripts, and meta tags. --> Texas A&M AgriLife Research Menu BLAST Tutorial Sequence similarity searching using NCBI BLAST This tutorial is designed to serve as a basic introduction to NCBI’s BLAST. The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between se

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