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Application Note: SP8 FALCON: a novel concept in fluorescence lifetime imaging enabling video-rate confocal FLIM

nature.com · 2,015 words · saved by 1 readers

The Leica SP8 FALCON (FAst Lifetime CONtrast) is a fast and completely integrated fluorescence lifetime imaging microscopy (FLIM) confocal platform. SP8 FALCON delivers video-rate FLIM with pixel-by-pixel quantification, thanks to a novel concept for measuring fluorescence lifetimes built on fast electronics and sensitive spectral hybrid detectors. Photon arrival times are recorded at count rates typical for standard confocal imaging. The system has ultra-short dead time and powerful built-in algorithms for data acquisition and analysis. The deep integration of FLIM into the confocal platform provides easy access to complex FLIM experiments.

Produced by Email Bluesky Facebook LinkedIn Reddit Whatsapp X Download PDF Confocal imaging is the current standard for fluorescence imaging in the life sciences. Functional imaging goes beyond the traditional recording of the location and concentration of molecular species, and enables further investigation of molecular function: their interactions with other biomolecules as well as their activity, conformation, molecular environment and post-translational modifications. Ideally, this must be accomplished at high spatiotemporal resolution. Fluorescence lifetimes are exquisitely suited to repo

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