More than double the fun with two-photon excitation microscopy | Communications Biology
This review introduces the basic principle of fluorescence and two-photon excitation microscopy that is aimed at novice and advanced microscopists highlighting current techniques, trends, and practical guides for live imaging optimization.
Download PDF Subjects Fluorescence imaging Multiphoton microscopy Abstract For generations researchers have been observing the dynamic processes of life through the lens of a microscope. This has offered tremendous insights into biological phenomena that span multiple orders of time- and length-scales ranging from the pure magic of molecular reorganization at the membrane of immune cells, to cell migration and differentiation during development or wound healing. Standard fluorescence microscopy techniques offer glimpses at such processes in vitro, however, when applied in intact systems, they
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