Role of competition between polarity sites in establishing a unique front | eLife

Polarity establishment in many cells is thought to occur via positive feedback that reinforces even tiny asymmetries in polarity protein distribution. Cdc42 and related GTPases are activated and accumulate in a patch of the cortex that defines the front of the cell. Positive feedback enables spontaneous polarization triggered by stochastic fluctuations, but as such fluctuations can occur at multiple locations, how do cells ensure that they make only one front? In polarizing cells of the model yeast Saccharomyces cerevisiae, positive feedback can trigger growth of several Cdc42 clusters at the same time, but this multi-cluster stage rapidly evolves to a single-cluster state, which then promotes bud emergence. By manipulating polarity protein dynamics, we show that resolution of multi-cluster intermediates occurs through a greedy competition between clusters to recruit and retain polarity proteins from a shared intracellular pool. The cells of fungi and other eukaryotic organisms are gen

Role of competition between polarity sites in establishing a unique front | eLife Version of Record January 12, 2016 Accepted Manuscript November 2, 2015 Download Cite Share Comment Open annotations (there are currently 0 annotations on this page). 2,696 views 646 downloads 69 citations Altmetric provides a collated score for online attention across various platforms and media. See more details Share this article Doi Copy to clipboard Cite this article Chi-Fang Wu Jian-Geng Chiou Maria Minakova Benjamin Woods Denis Tsygankov Trevin R Zyla Natasha S Savage Timothy C Elston Daniel J Lew (2015) R

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