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Shaping bacterial population behavior through computer-interfaced control of individual cells | Nature Communications

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Individual bacteria interact with each other and their environment to produce population-level patterns of gene expression. Here the authors use an automated platform combined with optogenetic feedback to manipulate population behaviors through dynamic control of individual cells.

Download PDF Subjects Microbial communities Computational platforms and environments Optogenetics Synthetic biology Abstract Bacteria in groups vary individually, and interact with other bacteria and the environment to produce population-level patterns of gene expression. Investigating such behavior in detail requires measuring and controlling populations at the single-cell level alongside precisely specified interactions and environmental characteristics. Here we present an automated, programmable platform that combines image-based gene expression and growth measurements with on-line optogene

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