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GFP Magnetofluorescence

andrewgyork.github.io · 4,035 words · saved by 1 readers

This project is maintained by Maria Ingaramo in the York lab, and was funded by Calico Life Sciences LLC Rebecca Frank Hayward, Julia R. Lazzari-Dean, Andrew G. York* and Maria Ingaramo† Calico Life Sciences LLC, South San Francisco, CA 94080, USA *Permanent email: andrew.g.york+magnetofluorescence@gmail.com †Permanent email: maria.del.mar.ingaramo+magnetofluorescence@gmail.com We've discovered a simple, nontoxic, biocompatible way to control the brightness of GFP-like fluorescent proteins via modest magnetic fields (~10 mT). Fluorescent proteins which seem magnetically inert (e.g. EGFP, mScarlet) become magnetoresponsive in the presence of an appropriate cofactor (e.g. EGFP-FlavinTag, or an mScarlet/FMN solution). This method works at room-temperature and body-temperature, in vitro, in E. coli and in cultured mammalian cells. The GFP-family magnetoresponse is weak ( Δ F / F ≈ 1 % Δ 𝐹 / 𝐹 ≈ 1 % ), but shows the hallmarks of evolvability. This suggests exciting technological possibili

Protein Magnetofluorescence You are using an outdated browser. Please upgrade your browser to improve your experience. Research article Magnetic control of the brightness of fluorescent proteins Rebecca Frank Hayward, Ashleen Rai, Julia R. Lazzari-Dean, Austin E.Y.T. Lefebvre, Andrew G. York * and Maria Ingaramo † Calico Life Sciences LLC, South San Francisco, CA 94080, USA * Permanent email: andrew.g.york+magnetofluorescence@gmail.com † Permanent email: maria.del.mar.ingaramo+magnetofluorescence@gmail.com Artwork, a generous gift by Alexey Chizhik Abstract We've discovered a sim

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