LITOS: a versatile LED illumination tool for optogenetic stimulation | Scientific Reports

Optogenetics has become a key tool to manipulate biological processes with high spatio-temporal resolution. Recently, a number of commercial and open-source multi-well illumination devices have been developed to provide throughput in optogenetics experiments. However, available commercial devices remain expensive and lack flexibility, while open-source solutions require programming knowledge and/or include complex assembly processes. We present a LED Illumination Tool for Optogenetic Stimulation (LITOS) based on an assembled printed circuit board controlling a commercially available 32 × 64 LED matrix as illumination source. LITOS can be quickly assembled without any soldering, and includes an easy-to-use interface, accessible via a website hosted on the device itself. Complex light stimulation patterns can easily be programmed without coding expertise. LITOS can be used with different formats of multi-well plates, petri dishes, and flasks. We validated LITOS by measuring the activity of the MAPK/ERK signaling pathway in response to different dynamic light stimulation regimes using FGFR1 and Raf optogenetic actuators. LITOS can uniformly stimulate all the cells in a well and allows for flexible temporal stimulation schemes. LITOS’s affordability and ease of use aims at democratizing optogenetics in any laboratory.

Download PDF Subjects Growth factor signalling Optogenetics Abstract Optogenetics has become a key tool to manipulate biological processes with high spatio-temporal resolution. Recently, a number of commercial and open-source multi-well illumination devices have been developed to provide throughput in optogenetics experiments. However, available commercial devices remain expensive and lack flexibility, while open-source solutions require programming knowledge and/or include complex assembly processes. We present a LED Illumination Tool for Optogenetic Stimulation (LITOS) based on an assembled

related reading

• Optogenetics - Wikipediaen.wikipedia.org
• A history of optogenetics: the development of tools for controlling brain circuits with light - PMCncbi.nlm.nih.gov
• All-Optical Interrogation of Neural Circuits - PMCncbi.nlm.nih.gov
• Wireless closed-loop optogenetics across the entire dorsoventral spinal cord in mice | Nature Biotechnologynature.com
• Gap Mapgap-map.org
• Optoception: perception of optogenetic brain stimulation | bioRxivbiorxiv.org
• Frontiers | Extracellular Optogenetics at the Interface of Synthetic Biology and Materials Sciencefrontiersin.org
• A blind man can make out objects again after an optogenetics treatment | MIT Technology Reviewtechnologyreview.com
• Three-dimensional scanless holographic optogenetics with temporal focusing (3D-SHOT) | Nature Communicationsnature.com
• Light sheet microscopy – Huisken Labhuiskenlab.com
• Award Details - NSF Award Searchnsf.gov
• Imaging localized fast optical signals of neural activation with optical coherence tomography in awake mice - PMCncbi.nlm.nih.gov