Establishment of myoblast cell line and identification of key genes regulating myoblast differentiation in a marine teleost, Sebastes schlegelii - ScienceDirect
Fig.1. Establishment and Characterization of skeletal muscle cell line from rockfish. The Morphologic observations of (A) Explant and cells derived from rockfish muscle tissue (5th, 7th, 10th and 15th day); (B) Subcultured muscle cells at passage 5, 10, 25 and 40. The cell line cultured by mixed medium has been successfully cultured more than 50 passages for over 150 days. Scale bar, 100 μm. (C) Growth curve of cells in mixed medium supplied with 15% FBS at 24 °C. The cell number was counted every day. Value are means ± SEM (n = 3). (D) The skeletal muscle cell line was characterized by amplification of the satellite cell-specific marker Pax7 and myoblast-specific marker MyoD at passage 5, 10, 25, 30, 40 and 50. β-actin were also amplified as internal control. Lane M is DNA marker. Fig. 2. The cells could differentiate into myotubes in vitro. (A) Morphological changes of cells after induction by differentiation medium. Myoblasts differentiated into myotubes (arrows) after induction. Sc