Transcriptomic insights into myogenic differentiation using muscle-derived cell lines with distinct myogenic potentials from black sea bream (Acanthopagrus schlegelii) - ScienceDirect
Fig. 1. Morphological and molecular characterization of myogenic and non-myogenic black sea bream cell lines during differentiation. (A) Representative phase-contrast images of myogenic and non-myogenic cell lines cultured in growth medium (GM) and differentiation medium (DM). Scale bars = 200 μm. (B) Representative fluorescence images of the cell lines stained with Calcein AM (cytoplasm, green) and Hoechst 33342 (nuclei, blue) after differentiation induction. (B1) The formation of multinucleated myotubes is clearly visualized in the myogenic cell line. (B2) Magnified view of the dashed box in B1, highlighting multiple nuclei (dashed circles) aligned within a single myotube. (B3) No multinucleated myotubes are observed in the non-myogenic cell line. Scale bars = 40 μm. (C) Fusion index of myogenic and non-myogenic cell lines after differentiation. The myogenic cell line exhibited a high fusion efficiency (average 33.5%) upon differentiation, whereas fusion events were not observed in t