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Continuous cultures using a turbidostat | Pioreactor Docs

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Determine the maximum specific growth rate of your culture using turbidostat strategies and pump extentions of the Pioreactor. Your Pioreactor can be transformed into a turbidostat, where pumps are used to replenish media around a target OD, creating a continuous culture. Due to the abundance of resources and lack of rate-limiting nutrients, the specific growth rate of a species is maximized. Learn more about the differences in bioreactor strategies here! This setup is a powerful tool that tests the limits of population growth. Students can investigate the specific maximum growth rate of a species and evaluate what innate biological processes are limiting growth. In our turbidostat system, we studied a small vial of YPD media inoculated with 4 drops of a yeast slurry. We set our volume to be exchanged to be 1 mL when the target OD was reached. This means at our target OD, 1 mL of media in the vial would be removed, and 1 mL of fresh media is added. We initially set our turbidostat targ

Success goal Determine the maximum specific growth rate of your culture using turbidostat strategies and pump extentions of the Pioreactor. Requirements ​ # One available Pioreactor Two peristaltic pumps: One media pump One waste pump Two liquid containers: One labelled "media" One labelled "waste" Scale to calibrate, if needed Introduction ​ Your Pioreactor can be transformed into a turbidostat , where pumps are used to replenish media around a target OD, creating a continuous culture. Due to the abundance of resources and lack of rate-limiting nutrients, the specific growth rate of a species

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