Comparing media dependent antimicrobial activity of random peptide mixtures in vitro and in mouse model - ScienceDirect
Fig. 1. The effect of LK20 RPM L:K ratio on bacterial growth. The tested bacteria (5 × 105 CFU/mL ) were incubated with serially diluted RPMs for 24 h at 37 °C in LB (A) or DMEM (B) media. Bacterial growth was measured at OD 595 nm in a plate reader (Tecan) and normalized against the growth of untreated bacteria. MIC values were defined as <20% growth. The results summarize three independent triplicates ± SEM. Fig. 2. The effect of 50:50 and 10:90 L:K ratio on membrane permeability and morphology: E. coli P4-NR (A-F) and S. aureus JLA513 (G-L), were incubated with LK20 at ratios of 50:50 or and 10:90 for 1 hour in LB (A-C,G-I) or in DMEM (D-F,J-L). The samples were washed 3 times in PBS, then suspended in 100 μL of 8% glutaraldehyde in PBS for 1 hour. Images captured using JEOL JSM-7800f-HR-SEM. Scale bar: 200 nm. Fig. 3. Comparing the RPMs interactions with bacterial membrane in LB and DEME media. Propidium iodide uptake by S. aureus (A) and E. coli (B) (106 CFU/mL) after 1 hour incub
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