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Protein purification workflow – The Bumbling Biochemist

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What’s a typical protein purification workflow? How do we select what stays and what goes? Is the prep going well? How can I tell? Disappearing bands on an SDS-PAGE gel I’m a protein biochemist so, as a “molecular mechanic” of sorts I study how proteins work (or don’t work). Which requires me to

May 27, 2019 January 7, 2023 What's a typical protein purification workflow? How do we select what stays and what goes? Is the prep going well? How can I tell? Disappearing bands on an SDS-PAGE gel I'm a protein biochemist so, as a "molecular mechanic" of sorts I study how proteins work (or don't work). Which requires me to have lots of pure protein, so I do a LOT of protein purification. I've done a lot of posts on various aspects of the process, but here I want to give you an overview of the entire process, mainly in graphics and more detail below. I'll hopefully be able to make a more polis

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