✳flâneur — a map of the web's best reading

Degenerate primers: Design and use

qiagen.com · 620 words · saved by 1 readers

Degenerate primers are mixtures of similar primer sequences that incorporate variations at specific positions to account for the degeneracy of the genetic code. This approach is useful when the precise nucleotide sequence of the target DNA is unknown but can be inferred from an amino acid sequence. PCR primer sequences are often deduced from amino acid sequences if the exact nucleotide sequence of their target is unknown. However, because of the degeneracy of the genetic code, the deduced sequences may vary at one or more positions. A common solution in these cases is to use a degenerate primer, which is a mixture of similar primers with different bases at the variable positions. Using degenerate primers can lead to difficulties in optimizing PCR assays:

Degenerate primers: Design and use PCR"> PCR > Introduction"> Introduction Guidelines for degenerate primer design and use Degenerate primers are mixtures of similar primer sequences that incorporate variations at specific positions to account for the degeneracy of the genetic code. This approach is useful when the precise nucleotide sequence of the target DNA is unknown but can be inferred from an amino acid sequence. PCR primer sequences are often deduced from amino acid sequences if the exact nucleotide sequence of their target is unknown. However, because of the degeneracy of the genetic c

Explore this link on the map →

related reading