Mutant forms of EGFR promote HER2 trafficking through efficient formation of HER2-EGFR heterodimers - ScienceDirect

Fig. 1. Detection of HER2 homodimers and heterodimers with an in situ PLA in cancer cell lines. Lung or breast cancer cell lines positive for HER2 amplification (amp) and WT for EGFR (A) as well as those without HER2 amplification and either negative (B) or positive (C) for EGFR activating mutations were subjected to a PLA (green signals) for detection of HER2 homodimers or HER2-EGFR or HER2-HER3 heterodimers. The representative images were obtained by optical sectioning. Nuclei (blue signals) were stained with 4′,6-diamidino-2-phenylindole (DAPI). Scale bars, 20 µm. Fig. 2. EGFR activating mutations promote HER2-EGFR heterodimerization in cancer cell lines without HER2 amplification. (A) z-Projection images were constructed by combination of optical sectioning images of multiple cells obtained by z-stacking. (B) Representative z-projection images obtained with an in situ PLA for HER2-EGFR heterodimers (green signals) in lung or breast cancer cell lines negative for HER2 amplification

Fig. 1. Detection of HER2 homodimers and heterodimers with an in situ PLA in cancer cell lines. Lung or breast cancer cell lines positive for HER2 amplification (amp) and WT for EGFR (A) as well as those without HER2 amplification and either negative (B) or positive (C) for EGFR activating mutations were subjected to a PLA (green signals) for detection of HER2 homodimers or HER2-EGFR or HER2-HER3 heterodimers. The representative images were obtained by optical sectioning. Nuclei (blue signals) were stained with 4′,6-diamidino-2-phenylindole (DAPI). Scale bars, 20 µm. Fig. 2. EGFR activating mu