Random antimicrobial peptide mixtures as non-antibiotic antimicrobial agents for cultured meat industry - ScienceDirect

Fig. 1. Antimicrobial activity of RPMs in tissue culture media. MIC values of various RPMs were examined against A) L. monocytogenes and B) E. coli NR. 107 and 106 CFU/mL of bacteria (respectively) were incubated with RPMs in DMEM for 24 h at 37 °C. Bacterial growth values were measured (595 nm) in a plate reader (Tecan) and normalized against the growth of untreated bacteria. The results summarize three independent triplicates ±SEM. A bacterial killing assay was conducted against C) L. monocytogenes and D) E. coli NR with an initial bacterial load of 106 CFU/mL. The bacteria were incubated with RPMs in DMEM for 1 h at 37 °C, then serially diluted in PBS and plated on LB-agar plates. The results summarize CFU count of three independent triplicates ±SEM. Fig. 2. Resazurin viability assay of MSCs after 24 h of incubation with RPMs. A) umbilical cord derived and B) Wharton jelly derived MSCs were incubated with RPMs for 24 h at 37 °C 5 % CO2. Cells viability values were calculated by fluo

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