AI-driven precision protease engineering for the food industry - ScienceDirect

Fig. 1. A new paradigm for modern protease engineering: synergy of data, knowledge, and intelligence. Note: This synergistic paradigm integrates three core sectors to enhance design efficiency and scalability beyond traditional single-strategy methods: Orange sector (left): Data-rich directed evolution explores vast sequence space via high-throughput mutant library screening and empirical fitness evaluation; Green sector (right): Knowledge-based rational design utilizes 3D structural and mechanistic insights for hypothesis-driven functional optimization; Blue sector (bottom): AI-driven predictive synergy employs deep learning to map sequence-function landscapes and pinpoint critical mutation sites, minimizing experimental effort; Overall Workflow: AI functions as the central integrator, continuously coupling experimental data with structural knowledge to guide iterative protein optimization and functional enhancement. (For interpretation of the references to colour in this figure legen

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• Rikard Saqe