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Fast and sensitive GCaMP calcium indicators for imaging neural populations | Nature

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Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript. Advertisement Nature volume 615, pages 884–891 (2023)Cite this article 149k Accesses 271 Citations 156 Altmetric Metrics details Calcium imaging with protein-based indicators1,2 is widely used to follow neural activity in intact nervous systems, but current protein sensors report neural activity at timescales much slower than electrical signalling and are limited by trade-offs between sensitivity and kinetics. Here we used large-scale screening and structure-guided mutagenesis to develop and optimize several fast and sensitive GCaMP-type indicators3,4,5,6,7,8. The resulting ‘jGCaMP8’ sensors, based on the calcium-bindin

Download PDF Subjects Fluorescent proteins Neural circuits Abstract Calcium imaging with protein-based indicators 1 , 2 is widely used to follow neural activity in intact nervous systems, but current protein sensors report neural activity at timescales much slower than electrical signalling and are limited by trade-offs between sensitivity and kinetics. Here we used large-scale screening and structure-guided mutagenesis to develop and optimize several fast and sensitive GCaMP-type indicators 3 , 4 , 5 , 6 , 7 , 8 . The resulting ‘jGCaMP8’ sensors, based on the calcium-binding protein calmoduli

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