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Label-Free Monitoring of Endometrial Cancer Progression Using Multiphoton Microscopy | Annals of Biomedical Engineering

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Endometrial cancer is the most common gynecological cancer in the developed world. However, the accuracy of current diagnostic methods is still unsatisfactory and time-consuming. Here, we presented an alternate approach to monitoring the progression of endometrial cancer via multiphoton microscopy imaging and analysis of collagen, which is often overlooked in current endometrial cancer diagnosis protocols but can offer a crucial signature in cancer biology. Multiphoton microscopy (MPM) based on the second-harmonic generation and two-photon excited fluorescence was introduced to visualize the microenvironment of endometrium in normal, hyperplasia without atypia, atypical hyperplasia, and endometrial cancer specimens. Furthermore, automatic image analysis based on the MPM image processing algorithm was used to quantify the differences in the collagen morphological features among them. MPM enables the visualization of the morphological details and alterations of the glands in the developm

Label-Free Monitoring of Endometrial Cancer Progression Using Multiphoton Microscopy Original Article Open access Published: 03 July 2024 Volume 52 , pages 3113–3124 ( 2024 ) Cite this article You have full access to this open access article Download PDF Save article View saved research Annals of Biomedical Engineering Aims and scope Submit manuscript Label-Free Monitoring of Endometrial Cancer Progression Using Multiphoton Microscopy Download PDF Abstract Endometrial cancer is the most common gynecological cancer in the developed world. However, the accuracy of current diagnostic methods is s

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