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SeqVerify: peace of mind for genome engineers

denovo.substack.com · 676 words · saved by 1 readers

Generating gene-edited cell lines has never been easier — over the last decade, a huge proliferation of tools (CRISPR, base editing, prime editing, engineered transposases and recombinases, etc) have allowed researchers to make defined genetic changes in order to answer biological questions. In my own work, I have extensively used CRISPR to create stem cell lines with fluorescent reporter alleles for lineage-specific marker genes. Basically this involves cutting the DNA at a specific site using CRISPR and introducing a repair template plasmid that the cell integrates at the cut site. The plasmid also contains a drug selection marker, which can be removed later on by adding a recombinase. These engineered stem cell lines will then become fluorescent when they differentiate to the correct cell type. But sometimes things can go wrong during the editing process: off-target mutations, unwanted mutations at the on-target site, chromosomal abnormalities, microbial contamination,1 or cell line

SeqVerify: peace of mind for genome engineers Metacelsus Sep 18, 2024 10 2 Share Generating gene-edited cell lines has never been easier — over the last decade, a huge proliferation of tools (CRISPR, base editing, prime editing, engineered transposases and recombinases, etc) have allowed researchers to make defined genetic changes in order to answer biological questions. In my own work, I have extensively used CRISPR to create stem cell lines with fluorescent reporter alleles for lineage-specific marker genes. Basically this involves cutting the DNA at a specific site using CRISPR and introduc

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