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Insights into non-crossover recombination from long-read sperm sequencing | bioRxiv

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Meiotic recombination is a fundamental process that generates genetic diversity by creating new combinations of existing alleles. Although human crossovers have been studied at the pedigree, population and single-cell level, the more frequent non-crossover events that lead to gene conversion are harder to study, particularly at the individual level. Here we show that single high-fidelity long sequencing reads from sperm can capture both crossovers and non-crossovers, allowing effectively arbitrary sample sizes for analysis from one male. Using fifteen sperm samples from thirteen donors we demonstrate variation between and within donors for the rates of different types of recombination. Intriguingly, we observe a tendency for non-crossover gene conversions to occur upstream of nearby PRDM9 binding sites, whereas crossover locations have a slight downstream bias. We further provide evidence for two distinct non-crossover processes. One gives rise to the vast majority of non-crossovers wi

Insights into non-crossover recombination from long-read sperm sequencing | bioRxiv Skip to main content New Results Insights into non-crossover recombination from long-read sperm sequencing View ORCID Profile Regev Schweiger , Sangjin Lee , View ORCID Profile Chenxi Zhou , Tsun-Po Yang , Katie Smith , Stacy Li , Rashesh Sanghvi , Matthew Neville , Emily Mitchell , Ayrun Nessa , Sam Wadge , Kerrin S Small , Peter J Campbell , View ORCID Profile Peter H Sudmant , Raheleh Rahbari , Richard Durbin doi: https://doi.org/10.1101/2024.07.05.602249 Regev Schweiger 1 Department of Genetics, University

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