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Dynamic assembly of the mRNA m6A methyltransferase complex is regulated by METTL3 phase separation | PLOS Biology

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Approximately one-fourth of eukaryotic mRNAs harbor at least one m6A-modified base, but how is this regulated? This study shows that cells can use liquid-liquid phase separation to regulate dynamic assembly of the mRNA m6A methyltransferase complex (METTL3/METTL14/WTAP), with stoichiometries that depend on condensate partitioning in a substrate binding-dependent manner.

Dynamic assembly of the mRNA m6A methyltransferase complex is regulated by METTL3 phase separation | PLOS Biology Article Authors Metrics Comments Media Coverage Reader Comments Figures Figures Abstract m 6 A methylation is the most abundant and reversible chemical modification on mRNA with approximately one-fourth of eukaryotic mRNAs harboring at least one m 6 A-modified base. The recruitment of the mRNA m 6 A methyltransferase writer complex to phase-separated nuclear speckles is likely to be crucial in its regulation; however, control over the activity of the complex remains unclear. Suppor

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