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Notes on embedding for brain preservation

brainpreservation.github.io · 17,473 words · saved by 1 readers

“Three rarely-discussed modes of preservation retain features of organisms more lifelike than any other kinds of fossils: freezing, dehydration, and preservation in amber.” - (Grimaldi et al., 1994) “It is manifest that flies, spiders, ants, and the like, that have accidentally been inclosed and buried in amber or even the gums of trees, never afterwards decay; though they are soft and tender bodies.” - Francis Bacon, The historie of life and death “There’s a great future in plastics.” - Mr. McGuire in The Graduate Embedding is the process of infiltrating biological tissue with a liquid medium that can then be solidified to provide structural support, enabling thin sectioning for microscopy. While the major purpose of embedding in electron microscopy is to allow for ultrathin sectioning and imaging, the embedding media themselves are potential options for long-term stabilization and storage of biological specimens for brain preservation. Key considerations for embedding agents in brain

Notes on embedding for brain preservation Notes on embedding for brain preservation By: Andrew McKenzie; Last Major Update: 2024-05-24; Status: Notes Quotes Summary Learning from nature: embedding in amber Structural preservation of biomaterials in amber Mechanisms of bioinclusion preservation in amber Definition of embedding Goals of resin embedding for electron microscopy vs brain preservation Allowing tissues to be sectioned easily Making tissues stable in the electron beam Chemistry of embedding agents Glueing of tissue morphology and biomolecules in place Solidification of embedding agent

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