Altair-dvOPM: an open-access platform for large-field three-dimensional tissue imaging | bioRxiv
Altair-dvOPM is an open-access direct-view oblique plane microscope designed for large-field, three-dimensional imaging of cleared and expanded tissue sections. By combining photographic-lens-based detection, externally launched oblique illumination and precision-registered modular baseplates, the system achieves micrometer-scale lateral resolution over a ~5.4 mm field of view without custom objectives or highly specialized alignment procedures. We demonstrate imaging across scales, from subcellular structures in expanded cells to centimeter-scale expanded tissue sections, and provide documentation, CAD files, Zemax models and open-source control software to support replication and extension. K.M.D. is a founder of Discovery Imaging Systems, LLC. K.M.D. and R.F. hold a patent on ASLM and have consultancy agreements with 3i, Inc. (Denver, CO, USA). H.Z. is a co-founder of Quotient Therapeutics and Jumble Therapeutics, and serves as an advisor to NewLimit. Reviews and Context 0 Comment 0
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