All-optical electrophysiology reveals brain-state dependent changes in hippocampal subthreshold dynamics and excitability | bioRxiv

A technology to record membrane potential from multiple neurons, simultaneously, in behaving animals will have a transformative impact on neuroscience research[1][1]. Parallel recordings could reveal the subthreshold potentials and intercellular correlations that underlie network behavior[2][2]. Paired stimulation and recording can further reveal the input-output properties of individual cells or networks in the context of different brain states[3][3]. Genetically encoded voltage indicators are a promising tool for these purposes, but were so far limited to single-cell recordings with marginal signal to noise ratio (SNR) in vivo [4][4]-[6][5]. We developed improved near infrared voltage indicators, high speed microscopes and targeted gene expression schemes which enabled recordings of supra- and subthreshold voltage dynamics from multiple neurons simultaneously in mouse hippocampus, in vivo . The reporters revealed sub-cellular details of back-propagating action potentials, correlations in sub-threshold voltage between multiple cells, and changes in dynamics associated with transitions from resting to locomotion. In combination with optogenetic stimulation, the reporters revealed brain state-dependent changes in neuronal excitability, reflecting the interplay of excitatory and inhibitory synaptic inputs. These tools open the possibility for detailed explorations of network dynamics in the context of behavior. [1]: #ref-1 [2]: #ref-2 [3]: #ref-3 [4]: #ref-4 [5]: #ref-6

All-optical electrophysiology reveals brain-state dependent changes in hippocampal subthreshold dynamics and excitability | bioRxiv Skip to main content New Results All-optical electrophysiology reveals brain-state dependent changes in hippocampal subthreshold dynamics and excitability View ORCID Profile Yoav Adam , Jeong J. Kim , Shan Lou , Yongxin Zhao , Daan Brinks , Hao Wu , Mohammed A. Mostajo-Radji , Simon Kheifets , Vicente Parot , Selmaan Chettih , Katherine J. Williams , Samouil L. Farhi , Linda Madisen , Christopher D. Harvey , Hongkui Zeng , Paola Arlotta , Robert E. Campbell , Adam

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