Plant-based hydrolysates as building blocks for cellular agriculture - ScienceDirect

Fig. 1. Commonly used cattle feeds for hydrolysis. Top to bottom; A) Cattle rearing pellets sourced from Barastoc, B) Dried Alfalfa grass C) Dried Kikuyu grass. Scale bar represents 4 cm. Fig. 2. The effect of plant-based hydrolysates with 0% FBS on growth of C2C12 cells. C2C12 cells were grown in the presence of selected hydrolysates, (1% v/v) cattle pellets, Alfalfa grass and Kikuyu grass in DMEM containing 0% FBS. A) A CTB reading taken at day three using fluorescence (Exc 560 nm/Em 590 nm) (* denotes significance compared to control p < 0.05) B) Cell confluency over 5 days measured by optical analysis, cell media was refreshed on the third day. Fig. 3. The effect of plant-based hydrolysates with 0.1% FBS on growth of C2C12 cells. C2C12 cells were grown in the presence of selected hydrolysates, (1% v/v) cattle pellets, Alfalfa grass and Kikuyu grass in DMEM containing 0.1% FBS A) A CTB reading taken at day three using fluorescence (Exc 560 nm/Em 590 nm) (* denotes significance compa

Fig. 1. Commonly used cattle feeds for hydrolysis. Top to bottom; A) Cattle rearing pellets sourced from Barastoc, B) Dried Alfalfa grass C) Dried Kikuyu grass. Scale bar represents 4 cm. Fig. 2. The effect of plant-based hydrolysates with 0% FBS on growth of C2C12 cells. C2C12 cells were grown in the presence of selected hydrolysates, (1% v/v) cattle pellets, Alfalfa grass and Kikuyu grass in DMEM containing 0% FBS. A) A CTB reading taken at day three using fluorescence (Exc 560 nm/Em 590 nm) (* denotes significance compared to control p < 0.05) B) Cell confluency over 5 days measured by opti