Induction of Apoptotic Program in Cell-Free Extracts: Requirement for dATP and Cytochrome c - ScienceDirect

Figure 3. Fractionation and Reconstitution of dATP-Dependent Activation of CPP32 by Phosphocellulose Chromatography Figure 4. Mono S Column Purification of Apaf-2 Table 1. Purification of Apaf-2 from HeLa Cells Figure 5. Absorption Spectrum of Apaf-2 Table 2. Sequences of Tryptic Peptides from the 15-kDa Apaf-2: Comparison with Human Cytochrome c Figure 6. Cytochrome c from Bovine Heart and Rat Livers Have Apaf-2 Activity Figure 7. Immunodepletion of Cytochrome c from HeLa S-100 and Reconstitution of dATP-Dependent Activation of CPP32, DNA Fragmentation, and Nuclear Morphological Change Using Purified Cytochrome c Figure 8. dATP and Cytochrome c–Dependent Activation of CPP32 in Cytosols from Human Embryonic Kidney 293 Cells and Human Monoblastic U937 Cells Figure 9. Reconstitution of dATP-Dependent Activation of CPP32 with S-cytosol and Purified Apaf-2 Figure 10. Increased Release of Cytochrome c to the Cytosol upon Apoptotic Stimulation All content on this site: Copyright © 2026 Elsev

Figure 3. Fractionation and Reconstitution of dATP-Dependent Activation of CPP32 by Phosphocellulose Chromatography Figure 4. Mono S Column Purification of Apaf-2 Table 1. Purification of Apaf-2 from HeLa Cells Figure 5. Absorption Spectrum of Apaf-2 Table 2. Sequences of Tryptic Peptides from the 15-kDa Apaf-2: Comparison with Human Cytochrome c Figure 6. Cytochrome c from Bovine Heart and Rat Livers Have Apaf-2 Activity Figure 7. Immunodepletion of Cytochrome c from HeLa S-100 and Reconstitution of dATP-Dependent Activation of CPP32, DNA Fragmentation, and Nuclear Morphological Change Using