Capacitation of human naïve pluripotent stem cells for multi-lineage differentiation | Development | The Company of Biologists

A.S. is an inventor on a patent application filed by the University of Cambridge relating to human naïve pluripotent stem cells. Maria Rostovskaya, Giuliano G. Stirparo, Austin Smith; Capacitation of human naïve pluripotent stem cells for multi-lineage differentiation. Development 1 April 2019; 146 (7): dev172916. doi: https://doi.org/10.1242/dev.172916 Download citation file: Human naïve pluripotent stem cells (PSCs) share features with the pre-implantation epiblast. They therefore provide an unmatched opportunity for characterising the developmental programme of pluripotency in Homo sapiens. Here, we confirm that naïve PSCs do not respond directly to germ layer induction, but must first acquire competence. Capacitation for multi-lineage differentiation occurs without exogenous growth factor stimulation and is facilitated by inhibition of Wnt signalling. Whole-transcriptome profiling during this formative transition highlights dynamic changes in gene expression, which affect many cell

A.S. is an inventor on a patent application filed by the University of Cambridge relating to human naïve pluripotent stem cells. Maria Rostovskaya, Giuliano G. Stirparo, Austin Smith; Capacitation of human naïve pluripotent stem cells for multi-lineage differentiation. Development 1 April 2019; 146 (7): dev172916. doi: https://doi.org/10.1242/dev.172916 Download citation file: Human naïve pluripotent stem cells (PSCs) share features with the pre-implantation epiblast. They therefore provide an unmatched opportunity for characterising the developmental programme of pluripotency in Homo sapiens.